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rabbit polyclonal anti lc3b  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal anti lc3b
    Rabbit Polyclonal Anti Lc3b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+lc3b/LC3B+Antibody+-+BSA+Free/pmc13011206-161-37-42
    Average 96 stars, based on 1654 article reviews
    rabbit polyclonal anti lc3b - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Article Title: HMG Box-Containing Protein 1 (HBP1) Protects Against Pancreatic Injury in Acute Pancreatitis but Promotes Neoplastic Progression.
    Article Snippet: Proteins were transferred to PVDF membranes (0.45 μm for standard proteins or 0.2 μm for small proteins) at 100 V for 2 hours in Tris/Glycine transfer buffer (Bio-Rad, 1610734). .. Membranes were blocked in 5% non-fat milk in TBST for 30 minutes and incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rabbit polyclonal anti-LC3B (NB600-1384, Novus Biologicals; 1:500) and rabbit monoclonal anti-SQSTM1/p62 (EPR4844, Abcam, ab109012; 1:1000). .. After washing with TBST, membranes were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG- HRP (Cell Signaling, 7076S; 1:2000) and goat anti-rabbit IgG-HRP (Cell Signaling, 7074S; 1:3000).

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis.
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4◦C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Control:

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Article Title: ATG5 paper_Revision PNAS_Avril 2023_Supplemental Figure 1_Final
    Article Snippet: Viral titers were determined by infection of HeLa cells with serial dilutions of the viral stocks for 24hrs, followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57fluorescein isothiocyanate (FITC) (Beckman Coulter). .. The following antibodies were used for immunoblotting and/or immunofluorescence: Mouse monoclonal anti-CAp24 HIV-1 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); ARP366), human monoclonal anti-SUgp120 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); 2G12), mouse monoclonal antiBST2 (Abnova; H00000684-M15), mouse monoclonal anti-GAPDH (Santa Cruz; sc-47724), mouse monoclonal anti-α-Tubulin (Sigma-Aldrich; T9026), mouse monoclonal anti-β-Actin (Sigma-Aldrich; A2228), mouse monoclonal anti-Flag (M2)-HRP (Sigma-Aldrich; A8592), rabbit polyclonal anti-ATG5 (Cell Signaling Technology; 12994S), rabbit polyclonal anti-LC3B (Novus Biologicals; NB600-1384), rabbit monoclonal anti-Syk (Cell Signaling; #12358), rabbit monoclonal anti-phosphoSyk (Cell Signaling; #2715), rabbit polyclonal anti-BST2 (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 11721) and rabbit polyclonal anti-Vpu (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 969), rat monoclonal anti-HA (3F10)-HRP (Roche; 12013819001), goat polyclonal anti-GFP-HRP (GeneTex; GTX26663). .. Anti-phosphoBST2 antiserum was elicited in rabbits by using a modified peptide of BST2 (amino acids 1 to 21) phosphorylated on tyrosines in position 6 and 8, generating a polyclonal antibody against the tyrosine-phosphorylated intracellular portion of BST2 (Proteogenix).

    Article Title: NRF2 deficiency impairs proliferation and survival of chicken primordial germ cells via oxidative stress, mitochondrial dysfunction and apoptosis.
    Article Snippet: .. The membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat milk for 2 h at room temperature, followed by overnight incubation at 4◦C with primary antibodies: rabbit polyclonal anti-NRF2 (1:2000, 16396-1-AP, Proteintech, Wuhan, China), rabbit polyclonal anti-LC3B (1:1000, NB100-2220, Novus Biologicals, Littleton, CO, USA), and rabbit polyclonal anti-GAPDH (1:1000, 10494-1-AP, Proteintech, Wuhan, China), which was used as the internal loading control for normalization. ..

    Western Blot:

    Article Title: ATG5 paper_Revision PNAS_Avril 2023_Supplemental Figure 1_Final
    Article Snippet: Viral titers were determined by infection of HeLa cells with serial dilutions of the viral stocks for 24hrs, followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57fluorescein isothiocyanate (FITC) (Beckman Coulter). .. The following antibodies were used for immunoblotting and/or immunofluorescence: Mouse monoclonal anti-CAp24 HIV-1 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); ARP366), human monoclonal anti-SUgp120 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); 2G12), mouse monoclonal antiBST2 (Abnova; H00000684-M15), mouse monoclonal anti-GAPDH (Santa Cruz; sc-47724), mouse monoclonal anti-α-Tubulin (Sigma-Aldrich; T9026), mouse monoclonal anti-β-Actin (Sigma-Aldrich; A2228), mouse monoclonal anti-Flag (M2)-HRP (Sigma-Aldrich; A8592), rabbit polyclonal anti-ATG5 (Cell Signaling Technology; 12994S), rabbit polyclonal anti-LC3B (Novus Biologicals; NB600-1384), rabbit monoclonal anti-Syk (Cell Signaling; #12358), rabbit monoclonal anti-phosphoSyk (Cell Signaling; #2715), rabbit polyclonal anti-BST2 (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 11721) and rabbit polyclonal anti-Vpu (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 969), rat monoclonal anti-HA (3F10)-HRP (Roche; 12013819001), goat polyclonal anti-GFP-HRP (GeneTex; GTX26663). .. Anti-phosphoBST2 antiserum was elicited in rabbits by using a modified peptide of BST2 (amino acids 1 to 21) phosphorylated on tyrosines in position 6 and 8, generating a polyclonal antibody against the tyrosine-phosphorylated intracellular portion of BST2 (Proteogenix).

    Immunofluorescence:

    Article Title: ATG5 paper_Revision PNAS_Avril 2023_Supplemental Figure 1_Final
    Article Snippet: Viral titers were determined by infection of HeLa cells with serial dilutions of the viral stocks for 24hrs, followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57fluorescein isothiocyanate (FITC) (Beckman Coulter). .. The following antibodies were used for immunoblotting and/or immunofluorescence: Mouse monoclonal anti-CAp24 HIV-1 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); ARP366), human monoclonal anti-SUgp120 (National Institute for Biological Standards and Control Centralized Facility for AIDS Reagents (NIBCS); 2G12), mouse monoclonal antiBST2 (Abnova; H00000684-M15), mouse monoclonal anti-GAPDH (Santa Cruz; sc-47724), mouse monoclonal anti-α-Tubulin (Sigma-Aldrich; T9026), mouse monoclonal anti-β-Actin (Sigma-Aldrich; A2228), mouse monoclonal anti-Flag (M2)-HRP (Sigma-Aldrich; A8592), rabbit polyclonal anti-ATG5 (Cell Signaling Technology; 12994S), rabbit polyclonal anti-LC3B (Novus Biologicals; NB600-1384), rabbit monoclonal anti-Syk (Cell Signaling; #12358), rabbit monoclonal anti-phosphoSyk (Cell Signaling; #2715), rabbit polyclonal anti-BST2 (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 11721) and rabbit polyclonal anti-Vpu (NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH; 969), rat monoclonal anti-HA (3F10)-HRP (Roche; 12013819001), goat polyclonal anti-GFP-HRP (GeneTex; GTX26663). .. Anti-phosphoBST2 antiserum was elicited in rabbits by using a modified peptide of BST2 (amino acids 1 to 21) phosphorylated on tyrosines in position 6 and 8, generating a polyclonal antibody against the tyrosine-phosphorylated intracellular portion of BST2 (Proteogenix).

    Blocking Assay:

    Article Title: HMG Box-Containing Protein 1 (HBP1) Protects Against Pancreatic Injury in Acute Pancreatitis but Promotes Neoplastic Progression.
    Article Snippet: Proteins were transferred to PVDF membranes (0.45 μm for standard proteins or 0.2 μm for small proteins) at 100 V for 2 hours in Tris/Glycine transfer buffer (Bio-Rad, 1610734). .. Membranes were blocked in 5% non-fat milk in TBST for 30 minutes and incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rabbit polyclonal anti-LC3B (NB600-1384, Novus Biologicals; 1:500) and rabbit monoclonal anti-SQSTM1/p62 (EPR4844, Abcam, ab109012; 1:1000). .. After washing with TBST, membranes were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG- HRP (Cell Signaling, 7076S; 1:2000) and goat anti-rabbit IgG-HRP (Cell Signaling, 7074S; 1:3000).

    other:

    Article Title: Polyclonal LC3B Antibodies Generate Non-Specific Staining in the Nucleus of Herpes Simplex Virus Type 1-Infected Cells: Caution in the Interpretation of LC3 Staining in the Immunofluorescence Analysis of Viral Infections.
    Article Snippet: Primary antibodies included the rabbit polyclonal anti-LC3B (2220SS; Novus biologicals, Littleton, CO, USA), rabbit polyclonal anti-LC3B (ab51520; abcam, Cambridge, UK), rabbit monoclonal anti-LC3B (ab192890; abcam, Cambridge, UK), rabbit polyclonal anti-LC3A (AP1805a-EV; Abcepta, San Diego, CA, USA), rabbit polyclonal anti-LC3C (SAB4200822; Sigma, Saint Louis, MO, USA), mouse monoclonal anti-ICP8 major DNAbinding protein (ab20194; abcam, Cambridge, UK), rabbit monoclonal anti-lamin B1 (12586; Cell Signaling Technology, Danvers, MA, USA), mouse monoclonal anti-α-tubulin (T5168; Sigma, Saint Louis, MO, USA), mouse monoclonal anti-β-actin-peroxidase antibody (A3854; Sigma, Saint Louis, MO, USA), Alexa Fluor 647–phalloidin (A-22287; ThermoFisher, Waltham, MA, USA).

    Article Title: Polyclonal LC3B Antibodies Generate Non-Specific Staining in the Nucleus of Herpes Simplex Virus Type 1-Infected Cells: Caution in the Interpretation of LC3 Staining in the Immunofluorescence Analysis of Viral Infections
    Article Snippet: Primary antibodies included the rabbit polyclonal anti-LC3B (2220SS; Novus biologicals, Littleton, CO, USA), rabbit polyclonal anti-LC3B (ab51520; abcam, Cambridge, UK), rabbit monoclonal anti-LC3B (ab192890; abcam, Cambridge, UK), rabbit polyclonal anti-LC3A (AP1805a-EV; Abcepta, San Diego, CA, USA), rabbit polyclonal anti-LC3C (SAB4200822; Sigma, Saint Louis, MO, USA), mouse monoclonal anti-ICP8 major DNA-binding protein (ab20194; abcam, Cambridge, UK), rabbit monoclonal anti-lamin B1 (12586; Cell Signaling Technology, Danvers, MA, USA), mouse monoclonal anti-α-tubulin (T5168; Sigma, Saint Louis, MO, USA), mouse monoclonal anti-β-actin-peroxidase antibody (A3854; Sigma, Saint Louis, MO, USA), Alexa Fluor 647–phalloidin (A-22287; ThermoFisher, Waltham, MA, USA).

    Staining:

    Article Title: Autophagy selectively clears ER in TNF-α-induced muscle atrophy
    Article Snippet: Cells were cultured on ibidi polymer coverslips (Ibidi 81156), fixed in 4% paraformaldehyde (PFA) and permeabilized with DPBS (Gibco, 14040-117) containing 0.4% Triton X-100. .. Proteins were stained with the following commercial antibodies: rat monoclonal anti-LAMP1-FITC (BioLegend, sc-121606 1:1000), rabbit polyclonal anti-LC3B (Novus Biologicals, NB100-2220 1:500), mouse monoclonal anti-MyHC (Sigma-Aldrich, M4276 1:1000), rabbit polyclonal anti-p62 (Invitrogen, PA5-20839 1:1000), rabbit monoclonal anti-Ub K63 (Sigma-Aldrich, 05-1308 1:1000), rabbit polyclonal anti-WIPI2 (Atlas Antibodies, HPA019852 1:400), DAPI (Thermo Fisher, 62249) goat anti-mouse IgG (H+L) secondary antibody AF647 (Thermo Fisher, A-21235) and goat anti-rabbit IgG (H+L) secondary antibody AF488 (Invitrogen, 10729174). .. To distinguish discrete ER-associated puncta from the granular cytosolic GFP-KDEL signal in ssGFP-KDEL C2C12 cells, puncta were quantified using ImageJ.



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